Explore finite and infinite imagery; conjugate planes, back focal plan manipulation, Abbe theory image formation, point spread functions, camera principles, fluorescence, and advanced fluorescence techniques. Build an optical microscope. One-and-one-half lecture hours and three laboratory hours a week for one semester. Only one of the following may be counted: Biomedical Engineering 381J (Topic: Biomd Eng Mcrscpy Fndtn Lab), 381J (Topic: Microscopy Fundamentals), 386J. Prerequisite: Graduate standing in engineering and consent of instructor; prior course in linear systems and signals.