Restricted to biochemistry majors. Theory of physical methods used in biochemistry and molecular biology, with a strong emphasis on macromolecular structure determination by X-ray crystallography and cryo-electron microscopy. Includes surface plasmon resonance, isothermal titration calorimetry, and biolayer interferometry. Three lecture hours a week for one semester. Biochemistry 364D and Chemistry 364D may not both be counted. Prerequisite: Biochemistry 339F and 370 with a grade of at least B and consent of instructor.